In vitro Shoot Multiplication and Rooting of Banana cv. Sabri

Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan

MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of abnormal shoots. After the first apical b...

Effects of Temperature and Season on In Vitro Establishment and Shoot Multiplication of Picea abies (L.) H. Karst

Plant propagation originated  from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput method due to the   removal of seasonal barriers, lower cost of production with higher yield wh...

The efects of diferent kinds and cocentrations of carbon sorbon source on in vitro shoot multiplication of persian wainut

Control of Shoot Tip Necrosis and Plant Death during in Vitro Multiplication of Pistachio Rootstock UCB1 (Pistacia integrima × P. atlantica)

Pistachio is one of the most important nuts and its mass production using tissue culture techniques is of great importance. Micro-propagation has many complications. Contamination is one of the critical limitations in the establishment stage. This study focused on different NaClO and HgCl2 concentrations and exposure times. Shoot tip necrosis (STN) is one of the most common problems during larg...

Effects of Liquid Medium on Rooting and Acclimation of Regenerated Microshoots of Banana (Musa sapientum L.) cv. Sagar

A regeneration protocol ensuring a high frequency rooting of micro-shoots derived from apical and axillary buds of suckers of banana cv. Sagar was achieved by using liquid medium. When the explants were cultured on MS medium supplemented with 0.5 mg/l each of BA, Kn and NAA, a large number of shoots developed. With the progression of the number of subcultures, shoot proliferation was enhanced. ...